Table 1 Resource table.

Anti-mouse CD16/32 Antibody

Biolegend

Cat# 101302

RRID: AB_312801

APC anti-mouse/human CD11b Antibody

Biolegend

Cat# 101212

RRID: AB_312795

Brilliant Violet 421™ anti-mouse TNF-α Antibody

Biolegend

Cat# 506327

RRID: AB_10900823

F4/80 Monoclonal Antibody (BM8), FITC

Invitrogen

Cat# 11-4801-82

RRID: AB_2637191

FITC anti-mouse CD14 Antibody

Biolegend

Cat# 123307

RRID: AB_940578

HRP Goat Anti-Mouse IgG H+L

Biotium

Cat# 20400-1mg

RRID: AB_3083795

HRP Goat Anti-Rabbit IgG (H+L)

Biotium

Cat# 20402-1mg

RRID: AB_3083796

HRP Goat Anti-Rat IgG

Sigma Aldrich

Cat# A9037-1ML

RRID: AB_258429

Mouse monoclonal anti-β-ACTIN

Sigma Aldrich

Cat# A1978

RRID: AB_476692

Mouse monoclonal anti-CHOP

Cell Signaling Technology

Cat# 2895

RRID: AB_2089254

Mouse monoclonal anti-phospho-IκBα (Ser32/36)

Cell Signaling Technology

Cat# 9246

RRID: AB_2267145

Mouse monoclonal anti-NF-κB p65

Santa Cruz

Cat# sc-8008

RRID: AB_628017

Mouse monoclonal anti-ZBP1

Adipogen

Cat# AG-20B-0010

RRID: 2490191

Rabbit monoclonal anti-BIP

Cell Signaling Technology

Cat# 3177

RRID: AB_2119845

Rabbit polyclonal anti-GPX4

Abcam

Cat# ab41787

RRID: AB_941790

Rabbit polyclonal anti-IκBα

Santa Cruz

Cat# sc-371

RRID: AB_2235952

Rabbit monoclonal anti-MIF

Cell Signaling Technology

Cat# 87501S

RRID: AB_2943242

Rabbit monoclonal anti-phospho-NF-κB p65 (Ser536)

Cell Signaling Technology

Cat# 3033

RRID: AB_331284

Rabbit monoclonal anti-phospho-MLKL (Ser345)

Cell Signaling Technology

Cat# 37333

RRID: AB_2799112

Rat monoclonal anti-MLKL

Millipore

Cat# MABC604

RRID: AB_2820284

2-Propanol

Roth

Cat# 9866.5

3,3′5,5′ tetramethylbenzidine (TMB)

Sigma Aldrich

Cat# 87748

30% H₂O₂

Sigma Aldrich

Cat# 95313

ABT737

Cayman Chemical

Cat# 11501-1

Acetic Acid (CH₃COOH)

Roth

Cat# 3738.2

Acetone

Sigma Aldrich

Cat# 179124

Acetonitrile hypergrade for LC-MS

Merck Millipore

Cat# 1.00029.2500

Amersham ECL Prime Western Blotting Detection Reagent

Cytiva

Cat# RPN2235

Ammonium carbonate

Merck Millipore

Cat# 207861-500G

Ammonium hydroxide solution

Merck Millipore

Cat# 221228-1L-A

Ammonium persulfate

Sigma Aldrich

Cat# 431532

Arg10 HCL

Silantes

Cat# 201604102

Benzonase HC

Merck Millipore

Cat# 71206

Birinapant

Selleck Chem

Cat# S7015

Bovine Serum Albumin (BSA)

Sigma Aldrich

Cat# A7030

BODIPY™ 581/591 C11

Invitrogen

Cat# D3861

Brefeldin A

Biolegend

Cat# 420601

CAA (2-Chloracetamid)

Merck Millipore

Cat# 79-07-2

cOmplete^™, EDTA-free Protease Inhibitor Cocktail

Sigma Aldrich

Cat# 4693132001

DMEM, high glucose, no glutamine, no phenol red

Thermo Fisher

Cat# 31053028

DMEM, high glucose, GlutaMAX™ supplement

Thermo Fisher

Cat# 61965059

Dimethyl sulfoxide (DMSO)

PAN Biotech

Cat# P60-36720100

Dithiothreitol (DTT)

VWR

Cat# 441496P

Doxycycline

VWR

Cat# J63805.06

DRAQ7

Biolegend

Cat# 424001

eBioscience™ Fixable Viability Dye eFluor™ 780

Invitrogen

Cat# 65-0865-14

EDTA (Versene), 1%, in PBS, without Ca2+ and Mg2+

Genexxon Bioscience

Cat# C4263.0100

Emricasan

Hölzel

Cat# HY-10396

Erastin

Biomol

Cat# 5449/10

Ethanol 99%, absolute

Roth

Cat# 9065.3

Ethylenediaminetetraacetic acid (EDTA)

VWR

Cat# 1084520250

Ferrostatin-1

Cayman Chemicals

Cat# 17729

Formic acid

Honeywell/Fluka

Cat# 607-001-00-0

ITS+1

Sigma Aldrich

Cat# I2521

LPS from E. coli, Serotype EH100 (Ra) (TLRGRADE™) (Ready-to-Use)

Enzo

Cat# ALX-581-010-L001

Lys8 HCL

Silantes

Cat# 211604102

Lysyl Endopeptidase (LysC)

WAKO

Cat# 129-02541

Methanol-LC-MS grade

VWR

Cat# 1.06035.2500

Murine His-TNF-alpha

Walczak lab

Kupka et al. [115]

ML210

Tocris

Cat# 6429/10

N, N, N′, N′-Tetramethylethylenediamine (TEMED)

Sigma

Cat# T9281

Necrostatin-1s

Abcam

Cat# ab221984

PBS, pH 7.4

Thermo Fisher

Cat# 10010056

Penicillium Streptomycin (P/S)

Sigma

Cat# P4333-100ML

PhosSTOP^™

Sigma

Cat# 4906837001

Ponceau S

Sigma

Cat# P3504

Protease inhibitor

Sigma Aldrich

Cat# 4693132001

Recombinant Murine M-CSF

Peprotech

Cat# 315-02

RIPA buffer

Thermo Fisher

Cat# 89901

ROTIPHORESE^® Gel 30

Roth

Cat# 3029.2

RPMI 1640 medium

Thermo Fisher

Cat# 21875091

RSL3

Selleck Chem

Cat# S8155

S63845

Selleck Chem

Cat# S8383

SILAC DMEM FBS Kit

Silantes

Cat# 282006500

Skim milk powder

VWR

Cat# A0830

Sodium Acetate Trihydrate (CH3COONa.3H2O)

VWR

Cat# 1062671000

Sodium Azide

Sigma

Cat# S2002-100G

Sodium Chloride (NaCl)

VWR

Cat# 1064045000

Sodium dodecyl sulfate (SDS)

Sigma Aldrich

Cat# L3771-100G

STY-BODIPY

Cayman Chemical

Cat# Cay27089-500

Sulfuric acid 10% (H₂SO₄)

VWR

Cat# 95441000

SuperSignal^TM West Femto Maximum Sensitivity Substrate

Thermo Fisher

Cat# 34095

Tamoxifen (4OHT)

Sigma Aldrich

Cat# H7904-5MG

TEAB

Sigma Aldrich

Cat# T7408

Tris

VWR

Cat# 1083872500

Tris-HCl

VWR

Cat# 648313-250

Triton x100

VWR

Cat# 1086031000

Trypsin-EDTA (%0.25)

Gibco

Cat# 25200056

Tween 20

VWR

Cat# 0777-1L

Urea

Sigma Aldrich

Cat# U1250

Valine-d8

CK Isotopes

Cat# DLM-488

8-OHdG ELISA

BioVision

Cat# K4160-100

Bicinchoninic acid (BCA) protein assay

Biorad

Cat# 774985

Click Chemistry Capture Kit

Jena Bioscience

Cat# CLK-1065

CytoTox 96® Non-Radioactive Cytotoxicity Assay

Promega

Cat# G1780

LunaScript RT SuperMix Kit

NEB

Cat# E3010L

Luna Universal qPCR Master Mix

NEB

Cat# M3003E

Mouse CXCL1/KC DuoSet ELISA

R&D Systems

Cat# DY453

Mouse CXCL2/MIP-2 DuoSet ELISA

R&D Systems

Cat# DY452

Mouse IL-1beta/IL-1F2 DuoSet ELISA

R&D Systems

Cat# DY401

Mouse IL-6 DuoSet ELISA

R&D Systems

Cat# DY406

Mouse MIF DuoSet ELISA

R&D Systems

Cat# DY1978

Mouse TNF-alpha DuoSet ELISA

R&D Systems

Cat# DY410

NucleoSpin RNA Kit

Macherey-Nagel

Cat# 740955250

Prostaglandin E2 Parameter Assay Kit

R&D Systems

Cat# KGE004B

Proteome Profiler Mouse XL Cytokine Array

R&D Systems

Cat# ARY028

RealTime-Glo™ Extracellular ATP Assay

Promega

Cat# GA5010

Proteomics Data

PRIDE database

PXD050979

Lipidomics

Metabolights database

MTBLS9798

Metabolomics

National Metabolomics Data Repository [116]

ST003635

Bulk RNA seq

NCBI Gene Expression Omnibus

GSE262664

GPX4 Control mouse small cell lung cancer tumor line RP252.7

Bebber et al. [35]

N/A

GPX4 KO mouse small cell lung cancer tumor line RP252.7

Bebber et al. [35]

N/A

Tamoxifen-inducible GPX4 KO mouse embryonic fibroblast cell line Pfa1

Seiler et al. [31]

N/A

mFSP1 overexpressing, tamoxifen-inducible GPX4 KO mouse embryonic fibroblast cell line Pfa1

Doll et al. [9]

N/A

Immortalized murine bone marrow-derived macrophages (iBMDM line CL13)

De Nardo et al. [65]

N/A

ZBP1i MEFs

Jiao et al. [36]

N/A

Empty vector-inducible MEFs

Jiao et al. [36]

N/A

pBMDMs

von Karstedt lab

This paper

PMLFs

von Karstedt lab

This paper

Mus Musculus: C57BL/6 N in-house strain was used for the generation of primary cells

CECAD in vivo research facility at the Cologne University Medical Center

Jackson Laboratory Cat# 005304

RRID:

IMSR_JAX:005304

Please refer to Supplementary Table 6

MaxQuant versions 1.5.3.8 and 2.0.3

Tyanova et al. [100]

https://cox-labs.github.io/coxdocs/maxquant_instructions.html

Perseus 1.6.15

Tyanova et al. [101]

https://cox-labs.github.io/coxdocs/perseus_instructions.html

Uniprot reference genome

Last access 26.08.2020

The UniProt consortium

https://www.uniprot.org

ID: UP000000589

https://www.uniprot.org/proteomes/UP000000589

Tracefinder version 5.0

Thermo Fisher

https://www.thermofisher.com/order/catalog/product/OPTON-31001

Compound Discoverer version 3.2

Thermo Fisher

https://www.thermofisher.com/order/catalog/product/OPTON-31055

Flowjo version 10.6.2

BD Life Sciences

https://www.flowjo.com/solutions/flowjo/downloads/previous-versions

The FUSION Solo S software

Vilber

https://www.vilber.com

SeeMS version 3.0.23277.0

Chambers et al. [117]

https://proteowizard.sourceforge.io/download.html

Prism version 10

GraphPad

https://www.graphpad.com

ChemDraw version 21.0

Revvity Signals/Perkin Elmer

RRID:SCR_016768

https://revvitysignals.com/products/research/chemdraw

Skyline software 23.1

Chambers et al. [117]

https://proteowizard.sourceforge.io/download.html

IncuCyte 2021B

Sartorius

https://www.sartorius.com/en/products/live-cell-imaging-analysis/live-cell-analysis-software

AccuCor algorithm

Xiaoyang Su

https://github.com/lparsons/accucor