Fig. 2: Histopathological and Morphometric analysis of CS mice.
From: Cockayne syndrome mice reflect human kidney disease and are defective in de novo NAD biosynthesis
A Histology findings of different lesions in male CS mice compared to wild type (aged 17–21 weeks) and examples include (1) Cytomegaly; (2) Karyomegaly; (3) Hyaline casts; (4) Karyomegaly; (5) Tubular regeneration; (6) Tubular regeneration, Karyomegaly, and epithelial degeneration; (7) Karyomegaly and epithelial degeneration. Scale bar, 20 μm. B Histology findings of different lesions in CSA and CSB mice compared to wild type (aged 45–68 weeks) and examples indicated include (1) hyaline glomerulopathy; (2) cortical scarring; (3) tubular nuclear enlargement consistent with tubular injury and occasional apoptotic cells; (4) mitotic figures; (5) foci of plasma cell rich inflammation; (6) hyaline glomerulopathy; (7) tubular cytoplasmic vacuolation; (8) focal tubular epithelial karyomegaly; (9) bland hyaline casts. Scale bar, 60 µm. C Representative image (left) and quantification (right) of PSR staining in CS mice. Scale bar, 100 µm. D Mean glomerular area quantification. E Whole-slide H&E images with glomeruli color-coded according to their corresponding histogram bin by digital histology (refer to “Materials and Methods” for details). Tubular feature quantifications (F) Median Tubular area, (G) Median tubule eccentricity, and (H) Median Tubule Perimeter by digital histology. I p57 immunohistochemistry (IHC) in kidney sections of CSA and CSB mice. Representative IHC image (Top) and quantification (bottom) of p57 staining in CS mice is shown. Scale bar, 60 µm. J Representative and quantification of TUNEL staining in CS mice kidney sections. Scale bar, 60 µm. Data are presented as mean ± SD (n ≥ 3). A two‐way ANOVA Sidak test was used for the comparisons between males and females. p values indicated (Blue bar, WT; Red bar, HO CSA/CSB).
