Fig. 3: Inhibition of fatty acid synthesis ameliorated LPS-induced EC dysfunction in vitro. | Cell Death & Differentiation

Fig. 3: Inhibition of fatty acid synthesis ameliorated LPS-induced EC dysfunction in vitro.

From: Fatty acid synthesis promotes mtDNA release via ETS1-mediated oligomerization of VDAC1 facilitating endothelial dysfunction in sepsis-induced lung injury

Fig. 3

A, B HUVEC were pretreated with FASN inhibitor C75 for 30 min before LPS stimuli for 4 h. Protein levels of VCAM-1, E-selectin, PAI-1 and TF were analyzed by western blot (n = 3). C–I The mRNA levels of E-selectin, VCAM-1, ICAM-1, TF, PAI-1, MCP-1 and IL-8 with C75 treatment were detected by RT-qPCR (n = 3). J, K HUVEC were transfected with siRNA specific to FASN before LPS stimuli for 4 h. Protein levels of FASN, VCAM-1, E-selectin, PAI-1 and TF were analyzed by western blot (n = 3). L, M Immunoblot showing the expression levels of VCAM-1, E-selectin, PAI-1, and TF with PA (10 µM) or PAS (20 µM) (n = 3). All data were expressed as the mean ± SD. Comparison among three or more groups was analyzed by one-way ANOVA. ns no significance, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.

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