Fig. 1: Expression profiles of SEMA3G in GBM patients.

A The differentially expressed genes (DEGs) in various cell types (GSE162631). B Volcano plot of DEGs in endothelial cells (GSE162631). C Heatmap of SEMA3G expression in non-tumor and GBM tissue of different GEO data sets. D Bivariate correlation analysis of SEMA3G and markers for various types of cells (GSE162631). EC: endothelial cell; AC: astrocyte; MG: microglia; OL: oligodendrocyte; PC: pericyte; SMC: smooth muscle cell; FB: fibroblast. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, the P values were calculated by the Pearson correlation test. E SEMA3G expression profile in the various type of cells (GSE162631). F The expression level of SEMA3G in non-tumor brain (n = 10) and GBM tissues (n = 521) from the TCGA GBM dataset. ^***P < 0.001, as determined by the Mann-Whitney U test. G–I Expression levels of SEMA3G in non-tumor (from traumatic brain injury patients) and GBM tissues from patients. The relative mRNA level of SEMA3G were determined by qPCR (G). The representative bands (H) and quantification (I) of protein level of SEMA3G as determined by western blot analysis. Data are shown as mean ± s.e.m. n = 5, ^**P < 0.01, ^***P < 0.001, as determined by two-tailed unpaired t test. J The protein level of SEMA3G in the cerebrospinal fluid (CSF) of non-tumor (from traumatic brain injury patients) and GBM tissues assessed by ELISA. Data are shown as mean ± s.e.m. n = 5, ^**P < 0.01, as determined by two-tailed unpaired t-test.