Fig. 2: PLEKHA1-TACC2 stabilizes EphA2 by inhibiting EphA2 ubiquitination. | Cell Death & Differentiation

Fig. 2: PLEKHA1-TACC2 stabilizes EphA2 by inhibiting EphA2 ubiquitination.

From: The PLEKHA1-TACC2 fusion gene drives tumorigenesis via vascular mimicry formation in esophageal squamous-cell carcinoma

Fig. 2

A SDS/PAGE and silver staining analysis of proteins affinity purified by using anti-Flag beads from lysates of cells overexpressing Flag (vector) or Flag-Pe11Te17. EphA2 was one of the major interacting proteins identified by mass spectrum. B Co-immunoprecipitation assay using KYSE30 cell lysates with anti-Flag followed by immunoblotting with antibodies against the indicated proteins. C Results of co-immunoprecipitation assays and immunoblotting analyses of KYSE30 cells stably expressing Flag-tagged PLEKHA1, TACC2, Pe4Te20, Pe11Te17, Pe10Te23, Pe5Te17, Pe2Te23, or an empty vector and transfected with Myc-tagged EphA2 using the indicated antibodies. D The expression of EphA2 in KYSE30 cells stably expressing Flag-tagged PLEKHA1, TACC2, Pe4Te20, Pe11Te17, Pe10Te23, Pe5Te17, Pe2Te23, or an empty vector treated with CHX for indicated duration was determined by immunoblotting analyses. E KYSE30 cells stably expressing Flag-tagged PLEKHA1, TACC2, Pe4Te20, Pe11Te17, Pe10Te23, Pe5Te17, Pe2Te23, or an empty vector were transfected with Myc-tagged EphA2 and HA-cbl, and co-immunoprecipitation assays and immunoblotting analyses with the indicated antibodies were performed. F KYSE30 cells stably expressing Flag-tagged PLEKHA1, TACC2, Pe4Te20, Pe11Te17, Pe10Te23, Pe5Te17, Pe2Te23, or an empty vector were transfected with Myc-tagged EphA2 and HA-ubiquitin, and co-immunoprecipitation assays and immunoblotting analyses with the indicated antibodies were performed. G, H The expression of EphA2 in tumor tissues obtained from the ESCC patients (G) and PDX grown in mice (H). Scale bars were indicated in the images.

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