Fig. 4: GLUL competently binds to the TRIM25-SPRY subunit and UAP1. | Cell Death & Differentiation

Fig. 4: GLUL competently binds to the TRIM25-SPRY subunit and UAP1.

From: GLUL mediates FOXO3 O-GlcNAcylation to regulate the osteogenic differentiation of BMSCs and senile osteoporosis

Fig. 4: GLUL competently binds to the TRIM25-SPRY subunit and UAP1.The alternative text for this image may have been generated using AI.

A Molecular docking image of the TRIM25 protein and the GLUL/UAP1 protein. B The results of the Biacore assay to assess the binding strength of GLUL/UAP1 to TRIM25. C Representative co-IP images of TRIM25 with GLUL/UAP1 in HEK-293T cells. D Representative images of bimolecular fluorescence complementation experiments between TRIM25 carrying YFP (aa 1–154) and GLUL/UAP1 carrying YFP (aa 155–238). E Representative images of live-cell imaging after cotransfection of GFP-TRIM25, RFP-GLUL, and BFP-UAP1 into HEK293T cells. F Representative images of immunofluorescence staining for TRIM25, GLUL and UAP1 in BMSCs. G Schematic diagram of TRIM25 truncation. H Representative images of co-IP experiments conducted with Myc-UAP1, HA-GLUL, and Flag-TRIM25 full-length and truncated samples. **P < 0.01 vs. other groups; *P < 0.05 vs. other groups.

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