Fig. 1: TMAO exacerbates renal fibrosis in UUO mice. | Cell Death & Differentiation

Fig. 1: TMAO exacerbates renal fibrosis in UUO mice.

From: Intestinal metabolite TMAO promotes CKD progression by stimulating macrophage M2 polarization through histone H4 lysine 12 lactylation

Fig. 1: TMAO exacerbates renal fibrosis in UUO mice.The alternative text for this image may have been generated using AI.

In view of the high consistency among the same mouse species, 30 mice were divided into 6 groups, each consisting of 5 mice. The Normal group received no treatment, the Sham group underwent a laparotomy procedure, the Sham+TMAO group underwent the laparotomy procedure and received drinking water containing 0.3% TMAO, the UUO group underwent left kidney ureteral ligation, the UUO + TMAO group underwent left kidney ureteral ligation and received drinking water containing 0.3% TMAO, and the UUO + IMC group underwent left kidney ureteral ligation and received drinking water containing the TMAO inhibitor IMC at 0.06%, the intervention continued for 14 days. A The level of TMA, TMAO, Betaine, Creatinine, Carnitine, Choline in urine of different groups of mice. B Masson stain of the left kidney of different groups of mice. C α-SMA, collagen I, fibronectin, LDHA, PDH mRNA expression in the left kidney of different groups of mice. D Western blotting showing the α-SMA, collagen I, fibronectin, LDHA, PDH levels of the left kidney of different groups of mice. E IL-12, iNOS, IL-6, IL-1β, and TNF-α mRNA expression in the left kidney of different groups of mice. F Arg-1, Fizz1, YM-1, TGFβ1, MR, IL-10 mRNA expression in the left kidney of different groups of mice. G Elisa showing the Cystatin C, Creatinine, BUN, LD, Acetyl-CoA level in the serum, and the urine protein.

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