Fig. 1: DYRK2 regulates USP28 protein levels.
From: A novel feedback loop between DYRK2 and USP28 regulates cancer homeostasis and DNA damage signaling
A HEK-293T cells were transfected with a gradient of Flag-DYRK2. Samples were split for WB (upper panel) or RT-qPCR (lower panel; mean ± SD, n = 3). B HEK-293T cells were transfected with siRNA-control or siRNA-DYRK2 and the protein level of USP28 and DYRK2 analyzed by WB. C The protein level of the indicated proteins was analyzed in MDA-MB-468 WT or DYRK2 knockout cells by WB. D HeLa cells were transfected with Flag-USP28 WT or inactive mutant (C171A) in presence or absence of Flag-DYRK2 and protein levels were analyzed by WB. E, F HEK-293T cells were transfected with the indicated plasmid and protein levels were determined by WB. G HCT116 WT or FBXW7 knockout cells were transfected or not with HA-DYRK2 and protein levels were analyzed by WB. H Correlation between active DYRK2 (Y382 phosphorylation state) and USP28 protein abundance across CPTAC pan-cancer data. Each point represents a patient, colored by the cancer cohort. The Pearson correlation coefficient and corresponding P-value are displayed in the top left corner. I Bar plot showing the Pearson correlation coefficient between active DYRK2 (Y382 phosphorylation state) and USP28 protein abundance across individual cancer cohorts. A significant negative correlation was observed only in the lung adenocarcinoma cohort (P-value = 7e–5). Note: a representative blot is shown of at least 3 biological replicates.
