Fig. 6: DYRK2 interacts and co-localizes with USP28.
From: A novel feedback loop between DYRK2 and USP28 regulates cancer homeostasis and DNA damage signaling
A CHO cells were transfected with a GFP-DYRK2 plasmid, fixed and analyzed by confocal microscopy using GFP-fluorescence or indirect immunofluorescence with a USP28 antibody after being treated with 10 μM MG-132 with or without 10 μM Etoposide (ETP) for 6 h. DAPI was used to stain DNA. Overlapping localization is shown in yellow (DYRK2/USP28) or purple (DYRK2/USP28/DNA). The RGB profiles indicate fluorescence intensity of each signal through the white line. B HEK-293T cells were transfected with the indicated plasmids, Myc-DYRK2 was immunoprecipitated and USP28 levels were determined by WB. Analysis of the USP28-dependent stabilization effects on DYRK2 in HEK-293T cells transfected with different versions of Flag-DYRK2 deletion mutants (C) or point-mutants (D) as shown in the schematic representations (left panels). Note: a representative blot is shown of at least 3 independent biological replicates.
