Fig. 2: Brca1 and Brca2 mRNA downregulation in G0 depends on Lin37/DREAM and Rb.
From: BRCA1 and BRCA2 gene expression: p53- and cell cycle-dependent repression requires RB and DREAM
Wild-type (WT), Lin37-/- (Lin37-KO), Rb-/- (Rb-KO) or Rb-/-/Lin37-/- (DKO) NIH3T3 cells were used. Brca1 (A) and Brca2 (B) mRNA expression was measured from proliferating, density-arrested, or serum-starved cells. A, B Mean ± SD; n = 4, two-way ANOVA; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001. Analogous experiments were performed with serum-starved cells predominantly in G0 and cells serum-restimulated to re-enter the cell cycle. From G0 and restimulated cells, mRNA levels of Brca1 (C) and Brca2 (D) were analyzed. Maximal fold-changes were calculated as the ratio of the highest to lowest expression levels. Averages from two technical replicates each from four independent cell lines for each cell variant are shown. Expression was normalized to WT proliferating (A, B) or to maximum expression of WT (C, D).
