Fig. 6: ASXLs are required for the regulation of gene expression and ferroptosis by BAP1. | Cell Death & Differentiation

Fig. 6: ASXLs are required for the regulation of gene expression and ferroptosis by BAP1.

From: Epigenetic regulation of ACSL4 via H2A monoubiquitylation connects lipid metabolism to BAP1-mediated ferroptosis

Fig. 6

A–D Interactions between indicated proteins and BAP1 were verified by Western blotting in indicated cells (A, C). Western blotting analysis of indicated proteins in BAP1-WT and mutations UMRC6 cell lines (B, D). E Cell viability in indicated cells was measured after treatment with different concentrations of erastin for 30 h. F Bar graph showing cell death of the indicated cells upon 10 μM erastin treatment for 24 h. G Lipid peroxidation in the indicated cells after treatment with 10 μM erastin for 24 h was assessed by flow cytometry after C11-BODIPY staining. H Cell viability of indicated cells was measured after treatment with TBH (10 μM) for 6 h. Western blotting (I) and RT-PCR (J) analysis of ACSL4 expression in ASXL1-KO UMRC6 cells restored with BAP1. K Cell viability in indicated cells was measured after treatment with different concentrations of IKE for 24 h. L Bar graph showing cell viability in indicated cells treated with cystine limitation. Error bars are mean ± SD. All P values were calculated using a two-tailed unpaired Student’s t-test. n ≥ 3 independent repeats unless specified. ns not significant (P > 0.05).

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