Fig. 2: Single-cell expression profiling of non-malignant pleural specimens.
From: Fibroblast-like cells in mesothelioma can derive from tumor cells
A Study pipeline: samples obtained by video-assisted thoracic surgery were mechanically disaggregated to single cells. Viable cells were collected and processed by 10× Chromium Controller. B Uniform manifold approximation and projection (UMAP) of scRNA-seq data from n = 1 075 cells clustering in seven scRNA-seq groups, represented by different colors. C UMAP colored by sample origin. D Cell proportions in the 3 different samples of non-malignant pleura. E Heat map of expression of the top 5 marker genes for each of the 7 groups. Yellow: high expression; Purple: low/no expression. Each row represents a gene, and each column represents a single cell.
