Fig. 6: The JAB1/CRL4B complex co-transcriptionally represses the expression of PPARG and ACSL5.
From: JAB1/CRL4B complex represses PPARG/ACSL5 expression to promote breast tumorigenesis
A Venn diagram of upregulated genes from JAB1 RNA-seq and promoter genes from CUL4B ChIP-seq. B KEGG pathway analysis of the 1887 overlapping upregulated genes from JAB1 RNA-seq data and the promoter genes from CUL4B ChIP-seq data. C qRT-PCR analysis of mRNA expression of target genes in JAB1-knockdown MDA-MB-231 cells. D qRT-PCR analysis of mRNA expression of target genes in CUL4B-knockdown MDA-MB-231 cells. E qChIP analysis of the identified genes in MDA-MB-231 cells. F qChIP analysis of MDA-MB-231 cells on the promoters of target genes using antibodies against JAB1, CUL4B, or H2K119ub1. Results are expressed as fold change (FC) relative to control, with GAPDH as the negative control. G ChIP and Re-ChIP experiments in MDA-MB-231 cells. J1 JAB1, C4B CUL4B, D1 DDB1. (C–F) The error bars indicate the mean ± SD derived from three separate experiments; two-tailed unpaired t-test; *p < 0.05, **p < 0.01, ***p < 0.001.
