Fig. 5: Emergence of non-coding transcript biotypes is diminished in differentiating Down syndrome (DS) neurons. | Cell Death & Differentiation

Fig. 5: Emergence of non-coding transcript biotypes is diminished in differentiating Down syndrome (DS) neurons.

From: Single cell analysis of developing Merkel cells reveals the emergence of non-coding RNA biotypes as a hallmark of terminal differentiation

Fig. 5

A Top: schematic illustration showing the in vitro differentiation of induced pluripotent stem cells (iPSCs) established from healthy control (Ctrl) and Down syndrome (DS) embryos into neural precursor cells (NPCs) and neurons. Middle: schematic illustration showing the identification of differentially expressed (DE) transcripts upregulated in neurons relative to NPCs derived from control (left) and DS (right) iPSCs. Lower: Venn diagram showing the intersection of DE upregulated transcripts identified in control and DS neuronal differentiation. B Bar chart showing the relative proportion of transcript biotypes for differentially upregulated and all other transcripts uniquely identified in control or DS neuronal differentiation. All others, expressed transcripts not upregulated in neuron samples (N for Ctrl = 8798; N for DS = 9104). Comparison of protein-coding and non-coding proportions for transcripts upregulated in Ctrl neurons versus DS neurons: p = 7.63 × 10−11. C, D Top gene ontology terms associated with genes encoding differentially upregulated non-coding transcripts uniquely identified in control (C) or DS (D) neuronal differentiation. Bar magnitude, enrichment score; dot, p-value. See also Supplementary Fig. 14.

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