Fig. 7: TUFT1 knockdown suppresses the tumor-promoting effect of HSCs in mice. | Cell Death & Differentiation

Fig. 7: TUFT1 knockdown suppresses the tumor-promoting effect of HSCs in mice.

From: TUFT1 stabilizes TGF-β receptor II protein and facilitates activation of hepatic stellate cells into metastasis-promoting myofibroblasts

Fig. 7

A RNA sequencing revealed that TUFT1 knockdown suppressed HSC expression of tumor-promoting factors in response to TGF-β1 stimulation for 24 h. FDR P < 0.05. The minimum expression level (blue) to maximum expression level (red) is shown by a color bar. B WB confirmed that TGF-β1-stimulated expression of 4 tumor-promoting factors was suppressed by TUFT1 knockdown in HSCs. ***P < 0.001 by ANOVA, n = 3. C, D, E Subcutaneous co-injection of human HT29 CRC cells with either control or TUFT1 knockdown HSCs into nude mice revealed that the tumor-promoting effect of HSCs was reduced by TUFT1 knockdown. HT29 tumor growth curves are shown in (C). ***P < 0.001 by ANOVA, n = 6. The pictures of HT29 tumors at the endpoint are shown in (D), and tumor weights are shown in (E). ***P < 0.001 by ANOVA, n = 6. F α-SMA IF detected lower CAF densities in tumors arising from HT29+HSCshTUFT1 co-injections compared to control co-injections. Scale bar, 50 μm. ***P < 0.001 by ANOVA, n = 6. G WB showed that the levels of HSC-derived tumor-promoting factor were reduced in tumors arising from HT29 + HSC shTUFT1 co-injections compared to control co-injections. *P < 0.05, **P < 0.01, ***P < 0.001 by t-test, n = 6.

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