Fig. 6: Onc-p53 activates ATM signaling stabilizing itself, in-turn upregulating Cyclin A and Chk1 expression. | Cell Death & Differentiation

Fig. 6: Onc-p53 activates ATM signaling stabilizing itself, in-turn upregulating Cyclin A and Chk1 expression.

From: Oncogenic p53 induces mitotic errors in lung cancer cells by recopying DNA replication forks conferring targetable proliferation advantage

Fig. 6

A Immunoblot analysis to detect p53 levels in extracts of H1975 cells after cycloheximide chase at the indicated time points in the presence of vehicle (DMSO) or an ATM inhibitor, KU-600019, (ATMi). Actin was used as a loading control. B Bar graphs show densitometric analysis of total p53 levels normalized with loading control obtained from the immunoblot in (A). p value is shown at the bottom. C Immunoblot analysis showing Chk1 and Cyclin A expression in extracts from shGFP and shp53 H1975 cells at the early S phase in the presence of DMSO or ATMi. GAPDH was used as a loading control. Densitometric analysis of (D) Cyclin A and (E) Chk1 levels normalized with loading control obtained from the immunoblot in (C). Expression of (F) CCNA2 and (G) CHEK1 transcript levels normalized with GAPDH in shGFP and shp53 H1975 cells at the early S phase in the presence of DMSO or ATMi. The bar graphs (F, G) are plotted as mean ± SD of three independent experiments. H Proposed mechanisms of p53 accumulation and targeting of tumor growth.

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