Fig. 1: Validation of inducible apoptosis or necroptosis in engineered TC-1 tumor cells. | Cell Death & Differentiation

Fig. 1: Validation of inducible apoptosis or necroptosis in engineered TC-1 tumor cells.

From: Phagocytosis of necroptotic cells optimizes type 1 conventional dendritic cells for induction of a cytotoxic T-cell response

Fig. 1: Validation of inducible apoptosis or necroptosis in engineered TC-1 tumor cells.

A, B Graphical illustrations of signaling pathways involved upon cumate-induced overexpression of BimS leading to apoptosis A, or RIPK3 leading to necroptosis B. C Representative light microscopy images of indicated TC-1 cells, untreated or treated with cumate for 24 h. Blue arrowheads: apoptotic bodies; black arrowheads and magnified black inlay: membrane blebbing; yellow arrowheads: cell swelling; red arrowheads and magnified red inlay: dying cell morphology. Scale bar represents 40 µm. D–G Real-time Incucyte analysis of Cytotox Dye incorporation D, F and Caspase-3/7 activity E, G in TC-1-iBimS and TC-1-iRIPK3 cells during indicated treatments. Means ± SD of technical triplicates are shown, representative of two independent experiments.

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