Fig. 2: OVA-specific CD8+ T-cell proliferation induced by cDCs after phagocytosis of apoptotic or necroptotic TC-1-OVA cells. | Cell Death & Differentiation

Fig. 2: OVA-specific CD8+ T-cell proliferation induced by cDCs after phagocytosis of apoptotic or necroptotic TC-1-OVA cells.

From: Phagocytosis of necroptotic cells optimizes type 1 conventional dendritic cells for induction of a cytotoxic T-cell response

Fig. 2: OVA-specific CD8+ T-cell proliferation induced by cDCs after phagocytosis of apoptotic or necroptotic TC-1-OVA cells.

A Experimental design of the cDC-OT-I T cell co-culture platform. Created in BioRender. OVA-expressing TC-1 cells were treated overnight with cumate and cell debris (medium) was added at a 1:1 ratio of plated TC-1 cells to cDCs. As control, cDCs were loaded with 250 μg/ml sOVA. OT-I T cells and loaded cDCs were co-cultured at a 25:1 ratio. B Representative flow cytometry histograms showing CellTrace Violet (CTV) dilution in OT-I cells after 1, 2, 3, and 4 days of co-culture with indicated cDCs. Numbers in the histograms indicate CTV dilution peaks. C Frequency of OT-I cells in each CTV dilution peak after 4 days of co-culture with indicated cDCs. Division index D and proliferation index E of the OT-I cell population after 4 days of co-culture with indicated cDCs. Means ± SD of two independent experiments are shown in C and means ± SD of three independent experiments in D and E. **P < 0.01; one-way ANOVA with Tukey’s multiple comparisons test.

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