Fig. 5: Phagocytosis of apoptotic or necroptotic TC-1 tumor cells by cDC1s and cDC2s. | Cell Death & Differentiation

Fig. 5: Phagocytosis of apoptotic or necroptotic TC-1 tumor cells by cDC1s and cDC2s.

From: Phagocytosis of necroptotic cells optimizes type 1 conventional dendritic cells for induction of a cytotoxic T-cell response

Fig. 5: Phagocytosis of apoptotic or necroptotic TC-1 tumor cells by cDC1s and cDC2s.

A Experimental design of the phagocytosis assay tracking CellTracker Green (CTG)+ dead tumor cells by flow cytometry. Created in BioRender. TC-1 cells were treated overnight with cumate and cell debris (medium) was added at a 1:1 ratio of plated TC-1 cells to cDCs. B, C Representative flow cytometry contour plots (left panels) and bar graphs (right panels) showing the frequency of CTG+ cDC1s B and cDC2s C present in the phagocytosis assay at the indicated time points. Means ± SD of 3 to 4 independent experiments are shown. ns, not significant; *P < 0.05; **P < 0.01; two-way ANOVA with Tukey’s multiple comparisons test.

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