Fig. 4: miR-937-5p directly targets the tumor suppressor gene of FBXO16. | Cell Death & Disease

Fig. 4: miR-937-5p directly targets the tumor suppressor gene of FBXO16.

From: MIR937 amplification potentiates ovarian cancer progression by attenuating FBXO16 inhibition on ULK1-mediated autophagy

Fig. 4

a Venn diagram showing the overlapping 11 potential targets by lists of TSGs and targets of miR-937-5p acquired from TargetScan database. b The expression of 11 predicted genes were detected by qPCR analysis upon miR-937-5p mimics transfection in OVCAR3 cells. c Potential miR-937-5p binding sequence in the 3′ UTR of FBXO16 mRNA predicted by TargetScan. The sequence of the mutant construct for luciferase reporter assay was also indicated. d Relative luciferase activity was monitored after the former described reporter plasmids co-transfected with miR-937-5p mimics in HEK293T cells. The firefly luciferase activity was normalized to renilla activity. e FBXO16 protein level was detected following transfection of miR-937-5p mimics or inhibitors in OVCAR3 and A2780 cells. GAPDH was used as a loading control. f Xenograft tumors derived from OVCAR3 cells were displayed. Two cell lines of OVCAR3 with FBXO16 stable knockdown and one control cell line (vector) were s.c. injected into nude mice. g Growth curve for the xenograft tumors generated by OVCAR3 cells with stable knockdown of FBXO16. h Tumor weights were measured on day 21 after the mice being sacrificed. Data are shown as the mean ± SD. Similar results were obtained in three independent experiments. Statistical analysis was performed using two-tailed Student’s t-test in (b, d) and one-way ANOVA in (g, h); *P < 0.05; ***P < 0.001; ****P < 0.0001.

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