Fig. 2: Cytoplasmic DEVDase activity is induced by Lm infection in presence of TNF-α. | Cell Death & Disease

Fig. 2: Cytoplasmic DEVDase activity is induced by Lm infection in presence of TNF-α.

From: Executioner caspases degrade essential mediators of pathogen-host interactions to inhibit growth of intracellular Listeria monocytogenes

Fig. 2

HeLa cells were infected with Lm at a MOI of 1 in presence of TNF-a (A), left without any manipulations (B), or treated with STS (0.1 mg/ml) (C) for 7 h before staining the cells with FITC-DEVD-fmk for another hour. After fixation, the cells were counterstained with Hoechst and 647-phallodin, then analyzed by confocal microscopy. In all panels, bars are 20 μm. (D), DNA condensation, cytoplasmic and nuclear DEVDfitc was quantified by counting five visible fields (n ~ 50) in three independent experiments. Presented are average +/− SEM. Significant differences are indicated. P-values are * < 0.05, ** < 0.01 and *** < 0.005.

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