Fig. 1: Bacterial infection of murine macrophages leads to transcriptional and post-transcriptional downregulation of NEDD9. | Cell Death & Disease

Fig. 1: Bacterial infection of murine macrophages leads to transcriptional and post-transcriptional downregulation of NEDD9.

From: Macrophages downregulate NEDD9 to counteract S. Typhimurium- mediated FAK-AKT activation and lysosome inhibition

Fig. 1: Bacterial infection of murine macrophages leads to transcriptional and post-transcriptional downregulation of NEDD9.

A Heat map of microarray analysis of murine bone marrow-derived macrophages (mBMDMs) infected with S. Typhimurium (ST) for 2 hours compared to uninfected control macrophages (UI). Gene expression of UI and infected mBMDMs as arbitrary unit (AU), red for high expression, blue for low expression. N = 3, biological replicates. B STRING Analysis of functional network of the NEDD9 signaling axis for significantly altered gene expression of the analysis described in (A). Genes are highlighted by coloring them according to their fold-change in expression. C Real-time PCR analysis of Nedd9 levels normalized to GAPDH by calculation of Δ CT values from mBMDMs infected with ST, unpaired t-test: p (UI vs. 4 h) = 0.0424, experiment n = 3 with at least 3 technical replicates. D Western blot analysis of mBMDMs uninfected (UI) and infected with ST at indicated time points followed by (E) densitometric analysis of NEDD9 protein level normalized to GAPDH, n = 5, biological replicates, one-way ANOVA: p (UI vs. 1 h) = 0.0162, p (UI vs. 4 h) = 0.0001, p (UI vs. 24 h) < 0.0001. F Quantification of immunofluorescent staining of intrazellular NEDD9 protein, UI n = 9, ST 4 h n = 11, biological replicates, unpaired t-test: p = 0.0019. A p-value > 0.05 equals not significant (ns), *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001.

Back to article page