Fig. 3: Transcriptional changes upon TMZ exposure. | Cell Death & Disease

Fig. 3: Transcriptional changes upon TMZ exposure.

From: Therapy-induced senescence of glioblastoma cells is determined by the p21CIP1-CDK1/2 axis and does not require activation of DREAM

Fig. 3: Transcriptional changes upon TMZ exposure.

LN229 cells were exposed to 50 µM TMZ for 48 or 144 h. RNA was isolated and subjected to RNA-Seq. A The genes commonly up-regulated (upper panel) or down-regulated (lower panel) after 48 and 144 h exposure are displayed as Venn diagrams. B Significantly regulated pathways were identified by the Reactome pathway browser (https://reactome.org/PathwayBrowser/). C The up-regulated genes and overlap with p53 targets are displayed as Venn diagrams. D The down-regulated genes and overlap with DREAM targets are displayed as Venn diagrams. E LN229 cells were exposed to 50 µM TMZ for 48 or 144 h. Expression of WEE1, PLK1, CENPA, MYT1, BIRC5, AURKA, AURKB, CDC20 and CDH1 was measured by qPCR. Differences between the control and TMZ treatment were statistically analyzed using Student's t test (non labelled = not significant; *p < 0.001 and log2 fold change > 1.5 are included.

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