Fig. 6: CDX2-mediated suppression of Pol II–DSIF–PAF1C complex formation blocked by mutations of its homeodomain. | Cell Death & Disease

Fig. 6: CDX2-mediated suppression of Pol II–DSIF–PAF1C complex formation blocked by mutations of its homeodomain.

From: CDX1 and CDX2 suppress colon cancer stemness by inhibiting β-catenin-facilitated formation of Pol II–DSIF–PAF1C complex

Fig. 6

A LGR5 luc reporter activities (mean ± SD) relative to those of the pGL4.10-luc2 control upon expressing β-catenin-S33Y, PAF1C components, wt-Cdx2, and its homeodomain (HD) mutants. P-values were calculated using a Student′s t-test. IP assays showing the effects of wt-Cdx2 and its HD mutants on β-catenin-S33Y (β-Cat.)-facilitated complex formation: B SPT5-containing complexes with PAF1, TFIIS, and RPB5 or C TFIIS-containing complexes with SPT5, PAF1, and RPBs. In the IP assays, the indicated proteins were expressed along with FLAG-SPT5 (B) or FLAG-TFIIS (C).

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