Fig. 2: Metabolomic and transcriptomic analysis of paired ILC cell lines. | Cell Death & Disease

Fig. 2: Metabolomic and transcriptomic analysis of paired ILC cell lines.

From: Invasive lobular carcinoma: integrated multi-omics analysis reveals silencing of Argininosuccinate synthase and upregulation of nucleotide biosynthesis in tamoxifen resistance

Fig. 2

A Partial Least Squares Discriminant Analysis (PLS-DA) showing metabolic differences between parental and tamoxifen-resistant SUM44 cell pair. B Variable Importance in Projection (VIP) plot highlighting the top 15 metabolites driving the separation between the parental and resistant SUM44 cell pair. C PLS-DA showing metabolic differences between parental and tamoxifen-resistant MB134 cell pair. D VIP plot highlighting the top 15 metabolites driving the separation between the parental and resistant MB134 cell pair. E Overlap of altered pathways in parental vs. tamoxifen-resistant SUM44 and MB134 pairs, represented by impact scores. F Venn diagram illustrating the number of shared and unique pathways altered in SUM44 and MB134 cell pairs, indicating common metabolic changes associated with tamoxifen resistance. G Significantly deregulated KEGG pathways in parental vs. tamoxifen-resistant MB134 cells and H SUM44 cells as determined by RNA sequencing analysis of cell pairs in quadruplicate. I Venn diagram showing the overlap of 52 deregulated pathways between parental and tamoxifen-resistant pairs of SUM44 and MB134 cell lines. J Venn diagram showing the overlap of metabolomic and transcriptomic data, identifying three mutually deregulated pathways in SUM44 and MB134TAMR cells. K Venn diagram showing the overlap of 15 genes within the three mutually deregulated pathways. L Gene-metabolite interaction map illustrating interactions between three deregulated pathways. Circles denote genes and squares denote metabolites.

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