Fig. 4: TGM2 staining patterns in human glioblastoma tumours.

A Image from full section showing strong TGM2 expression near regions of necrosis (indicated with N). Regions without necrosis (lower left) show lower levels of TGM2 staining; B Necrotic region showing TGM2 positive cells surrounding the border of the necrotic region, similar to what was observed in the xenograft model. C–F Double immunofluorescence for CD68 and TGM2 in whole sections from patient tumours. For each image row, the left panel shows CD68, the middle panel shows TGM2, and the right panel shows the merged image. Except for E, each row shows a single focal plane image from the Z stack. Row C shows a region with macrophages and endothelial cells. Row D shows a higher magnification view of a tumour blood vessel with adjacent macrophages to illustrate differences in subcellular location. Row E shows a Z stack of the same region as B, emphasizing the co-localization of TGM2 and CD68 in macrophages. Row F shows an area adjacent to necrotic regions.