Fig. 2: AGR2 sensitizes PDAC to ferroptosis in vitro.

A Western blot analysis demonstrated expression of AGR2 protein in AGR2 knockout groups compared to control cells in Capan2, HPAC, and PANC1 cells; Quantification of AGR2 mRNA expression relative to GAPDH is shown beneath the blot. B Heatmap representation of dysregulated genes expression in AGR2 knockout groups compared to control cells of HPAC, Capan2, and PANC1 cells. C KEGG pathway enrichment analysis of differentially expressed genes in HPAC and Capan2 cells upon AGR2 knockout. D Quantitative analysis showed the cell death rate of Capan2 and HPAC cells with or without AGR2 knockout, treated with different concentrations of Erastin (0–20 µM) or RSL3 (0–20 µM). E BODIPYTM 581/591 C11 levels of Capan2 and HPAC cells with or without AGR2 knockout, treated with or without the Lip-1. F Quantification of BODIPY 581/591 C11 fluorescence in Capan2 and HPAC cells. G Representative bright-field images of HPAC and Capan2 cells treated with TBH to induce ferroptosis in control, AGR2 KO, and AGR2 KO + Lip1 groups. Scale bar, 250 µm. Data are presented as the mean of 3 independent experiments ± SEM. *P < 0.1, **P < 0.01, ***P < 0.001.