Fig. 6: TLS contribute to proliferation of PD-1+CD8+T cells. | Cell Death & Disease

Fig. 6: TLS contribute to proliferation of PD-1+CD8+T cells.

From: CCL20 secreted by KRT15high tumor Cells promotes tertiary lymphoid structure formation and enhances anti-PD-1 therapy response in HPV+HNSCC

Fig. 6: TLS contribute to proliferation of PD-1+CD8+T cells.

A Clustering analysis of CD8+T cell subsets in HPV+ and HPV−HNSCC based on scRNA-seq data. B Bubble plot of marker genes for T cell subsets. C Clustering analysis of HPV+HNSCC based on spatial transcriptomics (ST) data. The circled regions indicate the groups containing mature TLS. D Expression of T cell subsets signature genes. E Spatial distribution of PDCD1 (PD-1) in HPV+HNSCC ST data. F IHC detection of PD-1 expression in TLS. G Immunofluorescence detection of PD-1+CD8+T cell infiltration in the TLS regions. H Violin plot showing the expression of ENTPD1 (CD39) in T cell subsets based on scRNA-seq data. I Spatial distribution of PD-1+CD39+MKI67+CD8+T cells in HPV+HNSCC ST data. J Spatial expression of antigen presentation pathway-related genes in HPV+HNSCC. K Multiplex immunofluorescence visualization of immune cell accumulation in TLS compartments. L Immunofluorescence detection of MKI67+CD8+T cell, CD21+dendritic cells and CD4+T cells within TLS.

Back to article page