Fig. 5: Sec8 competitively binds the 2CARD domain of RIG-I with STUB1.
From: Sec8: a novel positive regulator of RIG-I in anti-RNA viral defense
A Immunoprecipitation assays investigated the interaction between Sec8 and MDA5 or RIG-I. B Immunoprecipitation assays investigated the interaction between Sec8 and STUB1. C Models for the RIG-I truncated mutant. D, E Immunoprecipitation and immunoblotting analysis of the interaction of STUB1 or Sec8 with the RIG-I truncated mutant. F Models for the STUB1 truncated mutant. G Immunoprecipitation and immunoblotting analysis of the interaction of Sec8 with the STUB1 truncated mutant. H, I Immunoprecipitation analysis of the interaction between STUB1 and RIG-I in Sec8-overexpressing or Sec8-silencing HeLa cells infected with VSV for 12 h. J Immunoprecipitation analysis of the interaction between STUB1 and RIG-I in STUB1-KO cells infected with VSV for 12 h with MG132 treatment.
