Fig. 4: Ox-mtDNA is released in LPS-primed rotenone-induced BV2 cells. | Cell Death & Disease

Fig. 4: Ox-mtDNA is released in LPS-primed rotenone-induced BV2 cells.

From: Mitochondrial DNA drives NLRP3-IL-1β axis activation in microglia by binding to NLRP3, leading to neurodegeneration in Parkinson’s disease models

Fig. 4: Ox-mtDNA is released in LPS-primed rotenone-induced BV2 cells.The alternative text for this image may have been generated using AI.

A ATP content in BV2 cells (n = 3). B, C mtROS (red) production detected by MitoSOX Red staining and Quantitation of mtROS (n = 3). Scale bar = 25 μm. D The extra-mitochondrial ss/dsDNA (green) in LPS (100 ng/mL) primed rotenone (0.1 μM) treated BV2 cells was detected by Laser confocal microscopy. Scale bar = 5 μm. E BV2 cells cytosolic DNA was isolated and purified using the method in the Methods and whole-cell and cytosolic proteins were blotted using the Lamin B, TFAM and α-Tublin antibodies. F Relative amounts of total mtDNA in cytosols of LPS (100 ng/mL) primed rotenone (0.1 μM) stimulated BV2 cells (n = 3). Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001.

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