Fig. 5: NLRP3-IL-1β axis is involved in LPS-primed rotenone-induced neuronal damage.

A, B Immunofluorescence staining and quantification of the fluorescence intensity of Iba-1 (green) in BV2 cells were determined (n = 3). Scale bar = 25 μm. C, D NLRP3 and IL-1β mRNA expression level in BV2 cells detected by RT-qPCR (n = 3). E–H NLRP3, cleaved-caspase-1 and cleaved-IL-1β protein expressions in LPS (100 ng/mL) primed rotenone (0.1 μM) induced BV2 cells were measured by western blotting (n = 3). I IL-1β levels in medium were quantified using ELISA kits (n = 3). J SH-SY5Y cells were incubated with the BCM from BV2 cells for 24 h and cell viability was measured with MTT assay and K TH protein expression was measured by western blotting (n = 3). L–O NLRP3, cleaved-caspase-1 and cleaved-IL-1β protein expressions in NLRP3-KD BV2 cells treated with LPS (100 ng/mL) and rotenone (0.1 μM) were measured by western blotting (n = 3). P SH-SY5Y cells were incubated with the BCM from NLRP3-KD BV2 cells for 24 h, and microscopic observation of morphological changes (arrowed) in SH-SY5Y cells, Scale bar = 100 μm, Q cell viability was measured with MTT assay, and R TH protein expression was measured by western blotting (n = 3). Data are presented as mean ± SEM *p < 0.05, **p < 0.01, and ***p < 0.001.