Fig. 7: Treatment of pharmacological unfolded protein response (UPR) inducers, thapsigarin and tunicamycin, leads to OE19 and OE33 cell death. | Cell Death & Disease

Fig. 7: Treatment of pharmacological unfolded protein response (UPR) inducers, thapsigarin and tunicamycin, leads to OE19 and OE33 cell death.

From: Identification of isoform switching events linked with esophageal adenocarcinoma patient survival informs novel prognostic and therapeutic targets

Fig. 7: Treatment of pharmacological unfolded protein response (UPR) inducers, thapsigarin and tunicamycin, leads to OE19 and OE33 cell death.

Viability results and representative fluorescent images (48 h) of A OE19 and B OE33 cells treated with thapsigarin and tunicamycin. Error bars represent the standard error of the mean. Immunoblot of key UPR proteins and their downstream factors in C OE19 and D OE33 cells, following thapsigarin or tunicamycin treatment. Significance was determined using ANOVA with Tukey’s post-hoc test for multiple comparisons between treatments. Within each time point, treatments were significantly different from a = Vehicle, b = 1 μM thapsigarin, c = 2 μM thapsigarin, d = 50 ng/mL tunicamycin, e = 250 ng/mL tunicamycin, f = 500 ng/mL tunicamycin. CL cleaved protein. Scale bar, 500 μm.

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