Fig. 2: Low PRKACB expression elevates metastatic ability of DGC cells. | Cell Death & Disease

Fig. 2: Low PRKACB expression elevates metastatic ability of DGC cells.

From: Loss of PRKACB facilitates metastasis of diffuse-type gastric cancer through RhoA signaling activation

Fig. 2: Low PRKACB expression elevates metastatic ability of DGC cells.The alternative text for this image may have been generated using AI.

A, B After knocking down or overexpressing PRKACB in SNU-668 cells, a scratch assay was performed in a culture dish, and the statistical analysis of cell migration index was conducted using Image J software. Migration index: cell migration area/original scratch area. C–F After knocking down or overexpressing PRKACB in SNU-668 and KATO III, cell migration and invasion assays were performed in transwell chambers, and the number of cells was analyzed statistically using Image J software. G Co-immunofluorescence staining for E-cadherin and F-actin in PRKACB-knockdown SNU-668 cells. Scale bar, 25 µm. H, I After knocking down or overexpressing PRKACB in SNU-668 and KATO III, western blot was conducted to detect EMT markers. scr: scramble; OE: over expression; ns: not significant; *p < 0.05; **p < 0.01; ***p < 0.001.

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