Fig. 8: MCP-1 promoted resistance to radiotherapy of prostate cancer via activation of JAK/STAT1 pathway.

A, B DNA damage repairs of DU145 and PC-3 cells at the indicated time following irradiation were detected using comet assays. C, D γ-H2AX foci formations of DU145 cells at 8 h and 24 h after irradiation were measured by immunofluorescence. E, F The tumor growth curves and G weight of c57BL6/J mice implanted with RM-1 cells, accompanied by further intratumoral injection with different drugs in vivo xenograft tumorigenicity model. H, I The tumor growth curves and J weight of c57BL6/J mice implanted with RM-1 cells, accompanied by further radiation and intratumoral injection with different drugs in vivo xenograft tumorigenicity model. K A working model illustrating a positive feedback loop mechanism between prostate cancer cells and macrophages. Data were presented as mean ± SD of at least three independent experiments. *P < 0.05 by repeated-measures ANOVA test in (F, I). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 by one-way ANOVA test in (B, D, G, J).