Fig. 6: MiR-125a-5p targets RNF44.

A Binding sites between miR-125a-5p and RNF44 were predicted using Targetscan website; B Targeting relationship between miR-125a-5p and RNF44 was confirmed using dual luciferase reporter gene assay; C RNF44 expression in SCC-9/DDP cells after transfection of miR-125a-5p inhibitor+si-NC or miR-125a-5p inhibitor+si-RNF44 was detected using RT-qPCR; D Viability of SCC-9/DDP cells was determined using MTT assay; E IC50 of SCC-9/DDP cells was determined using MTT assay; F apoptosis of SCC-9/DDP cells was detected using flow cytometry; G glycometabolism of SCC-9/DDP cells was analyzed; N = 3; *P < 0.05; Data were expressed as mean ± standard deviation; t-test or ANOVA was used to analyze data.