Fig. 2: PARP1 and PARylation in differentiating myoblasts are sensitive to changes in metabolism. | Cell Death Discovery

Fig. 2: PARP1 and PARylation in differentiating myoblasts are sensitive to changes in metabolism.

From: PARP1 mediated PARylation contributes to myogenic progression and glucocorticoid transcriptional response

Fig. 2: PARP1 and PARylation in differentiating myoblasts are sensitive to changes in metabolism.

A, B Western immunoblotting of differentiating myoblasts differentiated in ±nicotinamide riboside (NR) (0.5 mM) (representative of n = 4) probed for PAR, PARP1, MYOD, and Alpha-Tubulin. C, D Western immunoblotting of differentiating myoblasts differentiated in ± NAMPT specific inhibitor FK866 (50 nM) (representative of n = 4) probed for PAR, PARP1, Myogenin, and Alpha-Tubulin, ***P < 0.001. E, F Western immunoblotting of differentiating myoblasts differentiated in ±dexamethasone (1 µM) probed for PAR, PARP1, MYOD and Alpha-Tubulin (representative of n = 4, **P < 0.01). G, H C2C12 myoblasts differentiated in differentiation medium containing different glucose concentrations before lysate harvest and immunoblotted for PAR, PARP1, Hexokinase II, and Alpha-Tubulin (representative of n = 3, *P < 0.05). I, J Western immunoblotting of differentiating myoblasts differentiated in ±dexamethasone (1 µM), PARP inhibitor PJ34 (10 µM) or both in combination (representative of n = 3, *P < 0.05, **P < 0.01).

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