Fig. 5: PARP1-mediated PAR events on Day 1 impact the myogenic trajectory. | Cell Death Discovery

Fig. 5: PARP1-mediated PAR events on Day 1 impact the myogenic trajectory.

From: PARP1 mediated PARylation contributes to myogenic progression and glucocorticoid transcriptional response

Fig. 5: PARP1-mediated PAR events on Day 1 impact the myogenic trajectory.The alt text for this image may have been generated using AI.

A Schematic representation of the PARP inhibitor treatment protocol during C2C12 myoblast differentiation. B Heatmap representing differential abundance of proteins within samples differentiated in ±PARP1 specific inhibitor BYK204165 (10 µM) (Vehicle n = 7, BYK204165 n = 6). C Volcano plot of SWATH-LCMS lysates recovered from day 6 differentiated C2C12 myoblasts differentiated in ± PARP inhibitor PJ34 (10 µM). Differential protein abundance shown with downregulated proteins marked green and upregulated proteins marked red. D Myotube fusion index of differentiating myoblasts differentiated in ±PARP inhibitor PJ34 (10 µM) or ±PARP1 specific inhibitor BYK204165 (10 µM) (n = 3). Cells were fixed on days 1, 3, and 6 of differentiation. Upper panel shows days 1, 3, and 6 fusion index, lower panel shows day 6 fusion index. Each bar represents means ± S.D (n = 3) ***P < 0.001, **P < 0.01. E Representative photographs of Jenner-Giemsa stained differentiating myoblasts over days 1, 3, and 6 of differentiation (n = 3).

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