Fig. 6: PARP1 transcriptome in myoblasts. | Cell Death Discovery

Fig. 6: PARP1 transcriptome in myoblasts.

From: PARP1 mediated PARylation contributes to myogenic progression and glucocorticoid transcriptional response

Fig. 6: PARP1 transcriptome in myoblasts.The alt text for this image may have been generated using AI.

A qPCR of PARP1 in scrambled sequence controls (n = 5) and siRNA PARP1 transfected (siPARP1) C2C12 myoblasts (n = 4) ***P < 0.001. B Western immunoblotting of protein lysates collected from scrambled and siPARP1 C2C12 myoblasts (n = 5). C Quantification of scrambled and siPARP1 transfections by western blotting (n = 5). D qPCR of Scrambled control and siPARP1 cDNA for PARP1, PARP2/PARP2, PARG, NAMPT, and SIRT1 transcripts. Scrambled (n = 5) and siPARP1 (n = 4). E Representative volcano plot of differential gene expression following RNAseq of scrambled and siPARP1 C2C12 myoblasts. F Gene set enrichment analysis (GSEA) of pathways over and under-represented in RNAseq data of siPARP1 C2C12 myoblasts. G Enrichment plots of GSEA in siPARP1 C2C12 myoblasts for hypoxia, H myogenesis and, I TNFα via NF-κB.

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