Fig. 6: MP-HJ-1b and colchicine affect the ferroptosis pathway in HeLa cells.
From: A novel microtubule inhibitor promotes tumor ferroptosis by attenuating SLC7A11/GPX4 signaling
A Intracellular ROS measurement by flow cytometry after treatment with the two compounds. (Marker, BODIPYTM 581/591 C11). B MP-HJ-1b and colchicine increase Fe2+ concentrations in HeLa cells. (t tests, *P < 0.05). C MP-HJ-1b and colchicine increase the concentration of MDA in HeLa cells. (t tests, *P < 0.05). D MP-HJ-1b and colchicine decrease intracellular G-SH levels. (t tests, *P < 0.05). E Electron micrograph. (magnification, 30000×; red arrow, mitochondrion; red rectangle, nucleus). F Western blotting analysis for SLC7A11, GPX4, LC3A/B, RPL35, and MRPL28. Ferrostatin-1 (fer-1) is a ferroptosis inhibitor. G Schematic of the mechanism by which the two compounds promote ferroptosis.
