Fig. 2: Subacute B[a]P exposure induces ferroptosis and upregulates Plin4 expression in mouse hippocampal tissue.
From: Plin4 modulates lipid droplet accumulation and ferroptosis in neurons exposed to benzo[a]pyrene
a Experimental workflow illustrating the steps involved in the construction of the B[a]P subacute exposure mouse model, followed by behavioral experiments and gene expression analysis. b Motion trajectory of mice in the MWM experiment. c Motion trajectory of mice in the Y-maze experiment. d Comparison of escape latency in the MWM experiment. e Comparison of platform crossings in the MWM experiment. f Comparison of time spent in the target quadrant in the MWM experiment. g Comparison of the number of new arm entries in the Y-maze experiment. h Comparison of the time spent exploring new arms in the Y-maze experiment. i–l qPCR results showing the relative expression levels of Gpx4, Slc7a11, Ptgs2, and Plin4 in hippocampal tissue. m Total GSH levels in hippocampal tissue. n GSH-Px activity in hippocampal tissue. o MDA content in hippocampal tissue. p Fe²⁺ levels in hippocampal tissue. Data are presented as mean ± SEM(n = 6 mice per group, Ctrl and B[a]P groups) *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
