Fig. 5: Effect of Plin4 silencing on BPDE-induced LD accumulation and morphological changes in HT22 cells.
From: Plin4 modulates lipid droplet accumulation and ferroptosis in neurons exposed to benzo[a]pyrene
a Oil Red O staining images showing LD accumulation (red arrows) in HT22 cells under different treatments: si-NC, BPDE, and si-Plin4 + BPDE. b TEM images revealing ultrastructural changes in LDs (red arrows) in the same treatment groups. c Quantification of LD area (% of cytoplasmic area) based on Oil Red O staining images shown in (a). d Quantification of LD area (% of cytoplasmic area) based on TEM images shown in (b). All quantitative data correspond to the same treatment groups: si-NC, BPDE, and si-Plin4 + BPDE. Data are presented as mean ± SEM (n = 6); *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
