Fig. 1: Analysis of protein expression in the absence of separase activity.

A Bar plot displaying the top 10 enriched Gene Ontology (GO) categories among differentially expressed proteins. The x-axis represents the ratio of observed to expected proteins for each GO category (y-axis). Only categories with an FDR < 0.05 are shown. A complete list of differentially expressed proteins can be found in Supplementary Tables 1 and 2. B Interaction network analysis of proteins associated with the enriched GO terms “Muscle structure development” (green spheres) and “Chromatin Organization” (red spheres). A k-means clustering algorithm was applied to group proteins into distinct clusters. Dashed lines indicate interactions between proteins of different clusters. Known interactions retrieved from curated databases or experimental studies are depicted in light blue and light purple, whereas green, red, and blue lines represent predicted interactions based on genomic neighborhood co-occurrence, fusion events, and gene co-occurrence patterns across genomes, respectively. Yellow lines highlight proteins frequently mentioned together in literature, black lines indicate co-expression relationships, and violet lines denote protein homology.