Fig. 3: Separase interacts with lamins at the nuclear envelope and influences lamin localization.
From: An evolutionarily conserved role for separase in the regulation of nuclear lamins
A GST pull-down assay using total protein extracts from wild-type third instar larval brains with GST, GSTPIM, and GSTSSE as baits. Interaction with Dm0 was assessed by western blot using an anti-Dm0 antibody. Input (IN) represents 1/10 of total protein extracts. B Western blot analysis with anti-LamC antibody following GST pull-down from brain extracts using GST, GSTPIM, and GSTSSE as baits. Input (IN) represents 1/10 of total protein extracts. The asterisk indicates a non-specific band. C Immunofluorescence staining of intact nuclei from Or-R salivary glands using anti-LamC (green) and anti-SSE (red) antibodies. Co-localization is observed as yellow staining in the merged images (c and f). DNA is counterstained with DAPI (gray in d, e, and f).
