Fig. 6: Separase interacts with lamin A in human cells.
From: An evolutionarily conserved role for separase in the regulation of nuclear lamins
A Proximity ligation assay (PLA) using anti-lamin A (LamA) and anti-ESPL1 antibodies in human SV40-fibroblasts transfected with control siRNA (siCTRL) or ESPL1-specific siRNA (siESPL1). Non-transfected (NT) primary fibroblasts were also analyzed. Red dots indicate interactions between endogenous lamin A and ESPL1, with nuclei counterstained with DAPI (blue). Histograms depict the quantification of PLA dots per nucleus (***p < 0.0001; Student’s t test). Control PLA experiments were performed using a single primary antibody against either ESPL1 or lamin A alone. B Lamin A-ESPL1 PLA in human primary fibroblasts performed as described in (A). Co-immunoprecipitation assays for endogenous human ESPL1 and lamin A. SV40-fibroblast lysates (1 mg) pre-treated with benzonase were immunoprecipitated with 5 µg of either mouse anti-ESPL1 (C) or mouse anti-lamin A (D) antibodies. Western blot analysis was performed on precipitates (IP) and 30 µg of input lysates using specific antibodies against ESPL1 and lamin A. A mouse IgG control (5 µg) was included (IgG).
