Fig. 1: OPN is highly expressed in CRC patients and correlates with poor survival. | Cell Death Discovery

Fig. 1: OPN is highly expressed in CRC patients and correlates with poor survival.

From: Colorectal cancer-derived osteopontin rewires macrophages into a pro-metastatic M2 state via the PI3K/AKT/CSF1-CSF1R axis

Fig. 1

A OPN expression in 20 paired CRC and adjacent tissues from the GSE142279 dataset (Deseq2). B, C TCGA data via UALCAN: B OPN expression in human CRC tumor tissues versus normal tissues (left), and correlation between OPN levels and CRC clinical stages (middle and right). C Kaplan–Meier curve illustrating the relationship between OPN expression and overall survival in CRC patients. D–G Validation in clinical specimens from our institution. D Schematic workflow: postoperative colorectal cancer tissues, adjacent tissues, and peripheral blood were collected from treatment-naive CRC patients (exclusion criteria: prior neoadjuvant therapy), and peripheral blood was collected from healthy donors. E Analysis of OPN in 6 paired CRC and adjacent tissues using Limma, from microarray data. F qPCR of OPN mRNA expression levels in 23 paired CRC and adjacent tissues. G ELISA analysis of OPN protein expression levels in peripheral blood from healthy individuals (n = 10) and CRC patients at stages II–III (n = 13) and stage IV (n = 17) CRC patients. All data are shown as mean ± SD; *p < 0.05; ****p < 0.0001; TCGA The Cancer Genome Atlas, COAD colon adenocarcinoma, READ Rectum adenocarcinoma.

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