Fig. 5: OPN induces M2-like polarization of macrophages in CRC and promotes reciprocal migration. | Cell Death Discovery

Fig. 5: OPN induces M2-like polarization of macrophages in CRC and promotes reciprocal migration.

From: Colorectal cancer-derived osteopontin rewires macrophages into a pro-metastatic M2 state via the PI3K/AKT/CSF1-CSF1R axis

Fig. 5: OPN induces M2-like polarization of macrophages in CRC and promotes reciprocal migration.The alternative text for this image may have been generated using AI.

A Experimental schematics of macrophage polarization models: indirect co-culture using Transwell chambers (0.4-μm pores) with CRC cells ± OPN inhibitor Compound 11(20 μM) (model 1, left); direct polarization via conditioned medium (CM) exposure(model 2, right). PMA-differentiated THP-1 macrophages were analyzed by flow cytometry after 72 h. B Flow cytometry analysis of CD206 (top) and CD163 (bottom) expression on macrophages. C Transwell migration and invasion assays of CRC cells (SW480, HCT8) co-cultured with M2-TAMs CM (left), with migrated CRC cells counts (right). D Transwell migration assays of PMA-stimulated THP-1 macrophages co-cultured with CRC cells CM (SW480, HCT8) (left), with migrated macrophage counts (right). E Transwell migration assays of PMA-stimulated THP-1 macrophages co-cultured with conditioned medium(CM) from OPNhigh CRC cells treated with OPN inhibitor (20 μM) (DLD1, HCT8) (left), with quantitative migration results (right). All data are shown as mean ± SD; **p < 0.01; ***p < 0.001; ****p < 0.0001.

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