Fig. 5: Molecular characterization of zebrafish alkbh3 gene. | Cell Discovery

Fig. 5: Molecular characterization of zebrafish alkbh3 gene.

From: ALKBH3-dependent m1A demethylation of Aurora A mRNA inhibits ciliogenesis

Fig. 5

a Cloning of zebrafish alkbh3 by reverse transcription PCR. b, c Schematic comparison of ALKBH3 amino acid sequences from the indicated species. The conserved 2OG-Fe (II) oxygenase superfamily domains are shown in blue filled bars. The conserved amino acid residues D193 and D185 are indicated, respectively. d Reverse transcription PCR analysis of alkbh3 mRNA at the different embryonic stages in zebrafish. 18s rRNA was served as a loading control. e Whole-mount in situ hybridization of zebrafish alkbh3 mRNA at the indicated developmental stages in lateral view.

Back to article page