Fig. 1: Trmt61a Expression and its impact on ILC3s and intestinal microbial homeostasis.
From: tRNA m1A modification is essential for gut homeostasis and function of group 3 innate lymphoid cells
a Real-time RT-PCR analysis of Trmt61a mRNA levels in ILC3s isolated from the colonic lamina propria of Trmt61afl/fl (n = 4) and Trmt61aΔRorc mice (n = 5). b–d Flow cytometry analysis of ILC3s in colonic lamina propria lymphocytes (LPLs): representative plots (b); population frequency (c); cell counts (d). n = 5 mice per group. e–j Analysis of CD4+ and RORγt+ CD4+ T cells in colonic LPLs: e, h Representative flow cytometry plots. f, i Population frequencies. g, j Cell counts. n = 4 mice per group. k–m Counts of ILC3 subsets in colonic LPLs: k CCR6+ ILC3s. l Nkp46+ ILC3s. m DN (double-negative) ILC3s. n = 6 mice per group. n–p IL-22+ ILC3s in colonic LPLs: n Representative flow cytometry plots. o Population frequency. p Cell counts. n = 5 mice per group. Data are pooled from two independent experiments, presented as means ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.
