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Data availability
The corresponding coordinates and cryo-EM density map have been deposited in the Protein Data Bank (http://www.rcsb.org/pdb) with code 7WKD, and in EMDB (http://www.ebi.ac.uk/pdbe/emdb/) with code EMD-32565.
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Acknowledgements
We thank Kai Wu and Wen Hu from Advanced Center for Electron Microscopy at Shanghai Institute of Materia Medica, Chinese Academy of Sciences for providing technical support and assistance during data collection, Yuqi Ping from our laboratory for the suggestions and critical reading during manuscript preparation. This work was partially supported by the Ministry of Science and Technology of China (2018YFA0507002), the National Natural Science Foundation of China (32130022), Shanghai Municipal Science and Technology Major Project (2019SHZDZX02), Shanghai Municipal Science and Technology Major Project, CAS Strategic Priority Research Program (XDB37030103) to H.E.X.; Fund of Youth Innovation Promotion Association (2018319 Y8G7011009) to X.C.; Science and Technology Commission of Shanghai Municipal (20431900100) and Jack Ma Foundation (2020-CMKYGG-05) to H.J.; the National Natural Science Foundation (31770796) and National Science and Technology Major Project (2018ZX09711002) to Y.J.; the National Natural Science Foundation of China (81902085), and Key tasks of LG laboratory (LG202101-01-03) to Y.X.
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Y.X. prepared samples for cryo-EM grid preparation; Q.Y. performed cryo-EM data collection; Y.X. processed the cryo-EM data, built the model, and refined the structure model. H.C. conducted functional studies and data analysis; C.Y. prepared the figures, performed the structural analysis; X.H. carried out docking analysis; X.C. and H.J. were responsible for molecular docking studies and writing of methods; Y.J. participated in the supervision of this research, fund acquisition, structural analysis, and manuscript editing; H.E.X. and Y.X. conceived and supervised the project; H.E.X. and Y.X. prepared the manuscript with the input from all authors.
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Xu, Y., Cai, H., You, C. et al. Structural insights into ligand binding and activation of the human thyrotropin-releasing hormone receptor. Cell Res 32, 855–857 (2022). https://doi.org/10.1038/s41422-022-00641-x
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DOI: https://doi.org/10.1038/s41422-022-00641-x
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