Fig. 1: The identification of the novel peptidomes.
From: Comprehensive discovery and functional characterization of the noncanonical proteome
a Schematic overview of the peptide identification process. b Distribution of sORFs and genes on chromosomes (upper). The relative quantity relationships between sORF and gene (lower left), mRNA-derived sORF and mRNA gene (lower median), ncRNA-derived sORF and ncRNA gene (lower right) on each chromosome were shown. Each dot represents a chromosome. The correlations were characterized by the spearsman correlation coefficient. c Sunburst map of the genes grouped by the number of peptides they encode. d Sunburst map of sORFs grouped by the host transcript type of them. uORF: upstream ORFs; dORF: downstream ORFs. e Stacked bar plot for number and percentage of detected sORFs. f Start codon (ATG/CTG/GTG/TTG) usage of peptides in the RLNPORF vs those actually identified by MS. g Boxplot for coding potential score comparison of ORFs encoding classic proteins (included in Uniprot) and sORFs analyzed by CPAT. Data are presented as individual value and statistic analysis was performed using two-sided unpaired two-samples non-parametric Wilcoxon test, P < 2.2E−16. h Boxplot for conservation score comparison of ORFs encoding classic proteins (included in Uniprot) and sORFs analyzed by Phastcons. Data are presented as individual value and statistic analysis was performed using two-sided unpaired two-samples non-parametric Wilcoxon test, P < 2.2E−16. i Density map for amino acid length of sORF derived from ncRNA or mRNA. j Boxplot for conservation score comparison of sORFs derived from mRNA and ncRNA analyzed by Phastcons. Data are presented as individual value and statistic analysis was performed using two-sided unpaired two-samples non-parametric Wilcoxon test, P < 2.2E−16. k Boxplot for conservation score of sORFs < 100 aa derived from mRNA and ncRNA analyzed by Phastcons comparison. Data are presented as individual value and statistic analysis was performed using two-sided unpaired two-samples non-parametric Wilcoxon test, P < 2.2E−16. l Boxplot for conservation score comparison of sORFs > 100 aa and < 250 aa derived from mRNA and ncRNA analyzed by Phastcons. Data are presented as individual value and statistic analysis was performed using two-sided unpaired two-samples non-parametric Wilcoxon test, P < 2.2E−16.
